Relative Rates of Lysis of Staphylococcal Cell Walls by Lytic Enzymes from Various Bacteriophage Types

Lysis of staphylococcal mastitis pathogens by bacteriophage phi11 endolysin.

The Staphylococcus aureus bacteriophage phi11 endolysin has two peptidoglycan hydrolase domains (endopeptidase and amidase) and an SH3b cell wall-binding domain. In turbidity reduction assays, the purified protein can lyse untreated staphylococcal mastitis pathogens, Staphylococcus aureus and coagulase-negative staphylococci (Staphylococcus chronogenes, Staphylococcus epidermidis, Staphylococcu...

Lysis of Staphylococcus aureus cell walls by a soluble staphylococcal enzyme.

Enzyme preparations of Staphylococcus aureus were examined for their ability to solubilize (32)P-labeled cell walls of the parent organism. Enzymatic activity was observed in the growth medium, in soluble fractions, and associated with native cell walls. Enzyme associated with isolated cell walls could be inactivated with formaldehyde without reducing the susceptibility of the walls to the acti...

Lytic activity of the recombinant staphylococcal bacteriophage Φ

Reproducibility of staphylococcal bacteriophage types by use of different concentrations of bacteriophage.

A comparative investigation was carried out on the reproducibility of staphylococcal bacteriophage types at 100 routine test dilution (RTD) and at the conventional concentrations, RTD and 1,000 RTD. The comparison includes multiple typing of laboratory strains and duplicate isolates from hospital patients. No significant difference was found in the reproducibility of the types at the different ...

non-linear study of various slit shear walls in steel structures

seismic retrofit strategies have been developed in the past few decades following the introduction of new seismic provisions and the availability of advanced materials and methods. it can be observed that new approaches to deal with more lateral forces are more innovative and more energy absorbent. in line with this, there is a growing trend toward the use of steel shear walls as a system with ...